Data/hora: 19/05/2024 04:49:05

Biblioteca(s): 	Embrapa Agricultura Digital.	
Tipo da produção científica: 	Resumo em Anais de Congresso	
Data corrente: 	26/03/2009	
Data da última atualização: 	15/01/2020	
Autoria: 	FALCAO, P. R. K.; TEIXEIRA, K. R. dos.	
Afiliação: 	PAULA REGINA KUSER FALCAO, CNPTIA; KATIA REGINA DOS SANTOS TEIXEIRA, CNPAB.	
Título: 	Evaluation of point mutation in genes related to ketogluconic acid production by Gluconacetobacter diazotrophicus.	
Ano de publicação: 	2008	
Fonte/Imprenta: 	In: INTERNATIONAL CONFERENCE OF BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 4., 2008, Salvador. Proceedings... Salvador: AB³C, 2008.	
Páginas: 	Não paginado.	
Idioma: 	Inglês	
Notas: 	X-Meeting 2008.	
Conteúdo: 	Here, bioinformatic approach based on protein modeling was applied to two ORFs related to ketogluconates production, gluconate 5-dehydrogenase and 2-ketoglucone reductase. The ORFs were analyzed and identification of regions that play an important role in substrate binding/recognition presents an opportunity for stability prediction and design of mutagenesis strategy. Gluconate-5-dehydrogenase was modelled using as a 3D model the crystal structure of Gluconate 5-dehydrogenase from Thermotoga maritima (PDB ID: vl8) whereas crystal structure of a NDA-dependent p-glycerate dehydrogenase (PDB ID:1gdh) was used to model 2-ketogluconate reductase. The sequence identities for these proteins are 36% and 34%, respectively. Based on this in silico model and with the prediction performed with CUPSAT [4], it was proposed to construct the mutants His289Asn and Glu271Ser of 2-ketoglucone reductase.	
Palavras-Chave: 	Bioinformática; Gluconacetobacter diazotrophics; Oxidação da glicose; Produção de ácido.	
Thesagro: 	Fixação de Nitrogênio.	
Thesaurus Nal: 	Bioinformatics; genes.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Agricultura Digital (CNPTIA)	
Exemplares: 	CNPTIA - (12687-1) - UPC - RA - PP -  -  - 2009.00056 - DES
CNPTIA - (12687-2) - UPC - RA - DD -  -  -  - DIS